goat serum blocking solution (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc goat serum blocking solution
Goat Serum Blocking Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+serum+blocking+solution/pmc13050104-88-21-25
Average 86 stars, based on 1 article reviews

goat serum blocking solution - by Bioz Stars, 2026-09

86/100 stars

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Blocking Assay:

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: .. Paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval with citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203) and blocking with normal goat serum blocking solution (CST, Cat# 5425S). .. Human LUAD sections were stained with anti-CD68 (ABclonal, Cat# A23205), and murine lung tumor sections were stained with anti-F4/80 (ABclonal, Cat# A23788).

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Sections were deparaffinized and rehydrated, followed by heat-induced antigen retrieval using citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203). .. Endogenous peroxidase activity was quenched with 3% H 2 O 2 (Abcarta, Cat# PK001), and nonspecific binding was blocked with normal goat serum blocking solution (CST, Cat# 5425S). .. Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499 ), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1-AP).

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Sections were deparaffinized and rehydrated, followed by heat-induced antigen retrieval using citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203). .. Endogenous peroxidase activity was quenched with 3% H2O2 (Abcarta, Cat# PK001), and nonspecific binding was blocked with normal goat serum blocking solution (CST, Cat# 5425S). .. Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1- AP).

Article Title: The dosage of curcumin to alleviate movement symptoms in a 6-hydroxydopamine-induced Parkinson's disease rat model
Article Snippet: .. The key experimental steps included blocking the brain slices for 2 h with goat serum blocking solution, treating the brain slices with primary antibody (1:500 Tyrosine Hydroxylase E2L6M Rabbit mAb #58844, Cell Signaling Technology, USA), incubating at 4 °C for 12 h, then treating with the secondary antibody (1:500 Goat Anti-Rabbit IgG H&L Alexa Fluor® 488 preadsorbed, Abcam, UK), and incubating at room temperature for 2 h. The brain slices were mounted on slides and protected with a cover glass. .. Then, confocal laser scanning microscopy (LSM880+Fast Airyscan, Carl Zeiss AG, Germany) was performed to observe the fluorescence effect using a 20 × objective lens (scale bar = 100 μm), a filter with a hydrogen ion laser, and a 488-nm emission.

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: .. Paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval with citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203) and blocking with normal goat serum blocking solution (CST, Cat# 5425S). .. Human LUAD sections were stained with antiCD68 (ABclonal, Cat# A23205), and murine lung tumor sections were stained with anti-F4/80 (ABclonal, Cat# A23788).

Article Title: M1 linear ubiquitination of LKB1 inhibits vascular endothelial cell injury in atherosclerosis through activation of AMPK.
Article Snippet: Endothelial cell injury is confirmed to be the initial step in the atherosclerosis (AS) process.. Here, we tried to elucidate the role of liver kinase B1 (LKB1) and adenosine phosphate protein kinase (AMPK) in modulating vascular endothelial cells (VECs) in AS.. High-fat feed (HFD)-induced AS rat models were prepared and treated with AMPK activator A-769662 alone or combined with chloroquine.

Incubation:

Article Title: Olanzapine enhances early brain maturation through activation of the NODAL/FOXH1 axis
Article Snippet: .. Goat serum-blocking solution (Cell signaling; Cat. 5425) was added, and the sections were sealed at 20-25°C for 1 h. The sections were then incubated with primary antibodies diluted in SignalStain® Antibody Diluent (Cell signaling; Cat. 8112) for 16 h at 4°C and then with the secondary antibodies at 20-25°C for 1 h, followed by counterstaining with DAPI (Abcam, Cat. ab104139). ..

Activity Assay:

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Sections were deparaffinized and rehydrated, followed by heat-induced antigen retrieval using citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203). .. Endogenous peroxidase activity was quenched with 3% H2O2 (Abcarta, Cat# PK001), and nonspecific binding was blocked with normal goat serum blocking solution (CST, Cat# 5425S). .. Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1- AP).

Binding Assay:

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Article Snippet: Sections were deparaffinized and rehydrated, followed by heat-induced antigen retrieval using citrate antigen-retrieval buffer (pH 6.0; Servicebio, Cat# G1203). .. Endogenous peroxidase activity was quenched with 3% H2O2 (Abcarta, Cat# PK001), and nonspecific binding was blocked with normal goat serum blocking solution (CST, Cat# 5425S). .. Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1- AP).

Review